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My thoughts and learning experiences regarding cellular immunology, cytokine assays and biology in general.
Friday, March 12, 2004
Most of what I've learned about ELISPOT and immunology, I've learned through CTL literature and my own readings here and there. Someday, I'll have to post something about readers and analyzers, and the technology involved. The image processing requirements are rather interesting, to say the least.
Misc notes for myself...
B cells, or B cell lymphocytes: B cells spend their entire early life in the bone marrow. Upon maturity, theytravel throughout the bloodstream and lymph, looking for antigens with which they can interlock. Upon identifying an antigen, a B cell starts replicating itself. These cloned cells mature into antibody-manufacturing plasma cells.
On the other hand T cells, or T cell lymphocytes leave the marrow at any early age, and mature in the thymus. Here they are imprinted with critical information for recognizing “self” and “non-self” substances. Depending on the variety, T cells can kill invading pathogens (killer T cells), or they can detect antigens and secrete cytokines to mobilize other immune system reactions (helper T cells), or they can modulate the immunity response, to prevent it from going out of control (suppressor T cells).
B cells, or B cell lymphocytes: B cells spend their entire early life in the bone marrow. Upon maturity, theytravel throughout the bloodstream and lymph, looking for antigens with which they can interlock. Upon identifying an antigen, a B cell starts replicating itself. These cloned cells mature into antibody-manufacturing plasma cells.
On the other hand T cells, or T cell lymphocytes leave the marrow at any early age, and mature in the thymus. Here they are imprinted with critical information for recognizing “self” and “non-self” substances. Depending on the variety, T cells can kill invading pathogens (killer T cells), or they can detect antigens and secrete cytokines to mobilize other immune system reactions (helper T cells), or they can modulate the immunity response, to prevent it from going out of control (suppressor T cells).
